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Cell Lysate Microarrays (Reverse Microarrays) - From Cells to Protein Profiles

For multiplexed protein profiling in cells and tissues, a system comprising a GeSiM Nano-Plotter, Zeptosens planar waveguide based chips, and a ZeptoREADER sets the standard with respect to precision, sensitivity and throughput.

From tissue to data: The procedure of Zeptosens Cell Lysate Microarrays
  Method

 

Microarrays with individual cell lysate spots of a 130 µm diameter, representing 1 to 10 cell equivalents each, are being produced using the robust piezo-electric non-contact deposition method of the Nano-Plotter. Each array comprises 32 samples (cell lysates) spotted in duplicates at 4 concentrations, each individual array is probed with one protein specific antibody. Signals are generated by fluorescently labeled antispecies antibodies. Due to the extremely low sample consumption of the spotting process large numbers of replicate arrays can be produced and thus allow the efficient determination of substantial numbers of proteins.

The simple, robust and highly parallel architecture of the assay yields precise quantitative information and allow time course monitoring of protein expression and activation.
  Cell Lysates on the waveguide chip

The applicability of the ZeptoMARK CeLyA approach has been demonstrated among others by

  • Identification of disease relavent marker proteins in cultured cells, tissues, microdissected material as well as depleted serum and urine
  • Determination of dose efficacy and IC50 of drug candidates
  • Quantification of cell signalling pathway activation / inhibition with a precision of better than 20%
  • Monitoring phosphorylation changes on multiple kinases  

Equipment: Nano-Plotter NP1.2, Nano-Plotter NP2.0/E


Courtesy of Zeptosens, a Division of Bayer (Schweiz) AG, Benkenstrasse 254, 4108 Witterswil (CH)