Development of a Pipetting Station for the Parallel Synthesis of Peptide Ligands for Optical Markers (Joint Research Project "PLOMS")
Peptides are used by nature as active regulators and messengers in the human body. They combine high specificity with high affinity in molecular recognition processes, therefore they are ideally suited as drugs.
Peptide microarrays can be utilized for the identification of peptide sequences suitable for pharmaceutical or diagnostic applications as well as for the screening of enzymatic substrates or for enzyme inhibitors.
In a passed research project the Nano-Plotter is applied to the automated synthesis of peptide libraries on a variety of different solid supports such as glass microcapillary plates. The spatially addressable synthesis of different peptides on solid supports allows the subsequent screening of the peptide libraries by established assay techniques including optical screening by fluorescence methods. Only very few peptide sequences from a combinatorial library are typically suitable for the desired tasks, therefore it is highly desirable to produce only the minimal quantity of the candidate sequences necessary for screening. This saves costs and is environmentally friendly. For the realization of microreactions the Nano-Plotter is particularly suited, as it is capable to perform dosage steps in the microliter as well as in the nanoliter range. These dosage steps can be performed highly reproducibly at desired reaction coordinates in the nanoliter scale, a precondition which is indispensable for the generation of peptide arrays in the biochip format on glass surfaces.
|Customized version of Nano-Plotter NP2.0/E for peptid synthesis|
|Synthesis Station for automatic creation of substance libraries on resin membranes||Synthesis station for automatic creation of substance libraries on micro capillary chips
Equipment: Nano-Plotter NP2.0/E
Dr. Niels Röckendorf
Research Center Borstel - Leibnitz Center for Medicine and Biosciences
LG Mucosal Immunology
Phone: +49 4537 188 457